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Attribute
Research Imaging & Microscopy

RELION-5

MRC Laboratory of Molecular Biology (Scheres Lab)
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CategoryResearch Imaging & Microscopy
CompanyMRC Laboratory of Molecular Biology (Scheres Lab)
PriceFree Open Source
StatusStatus not confirmed
AvailabilityAvailable on GitHub and CCP-EM
Last checked20/09/2026
FeaturesEmpirical Bayesian algorithms for 2D class averaging and 3D reconstruction, High-resolution CTF refinement and per-particle beam-induced motion correction, GPU acceleration for processing massive multi-terabyte movie datasets, Integrated model-building and validation tools for macromolecular complexes
Entry typeScientific Software
Access modeOpen Source
AI roleBayesian Image Reconstruction
Input dataNot recorded
Output dataNot recorded
Licence conditionsGPL-3.0
Commercial eligibilityOpen source release
Compute requirementsMulti-GPU workstation or cluster (NVIDIA GPUs with >= 16GB VRAM, fast NVMe scratch storage)
ValidationNot recorded
TypeCryo-EM image processing suite
Intended useNot recorded
CompatibilityNot recorded
ManufacturerMRC Laboratory of Molecular Biology
Biological applicationAtomic-resolution macromolecular structure determination (proteins, viruses, ribosomes)
Research workflowCryo-EM movie micrographs -> motion correction -> particle picking -> 3D density map
Evidence levelPrimary software used for thousands of atomic structures deposited in the Protein Data Bank (PDB)
Integration evidencehttps://relion.readthedocs.io
Laboratory handoffGenerates 3D density maps for atomic model building and drug binding validation